waskington state lake images
WATERLINE - September, 2016

Lake Tanganyika: Fish diversity, nutrient recycling and periphyton productivity in the littoral zone

Adapted from an article by Dr. Yvonne Vadeboncoeur
People collecting water from Lake Tanganyika

RUMONGE, BURUNDI: People collecting water from Lake Tanganyika in Rumonge – Photo: Giulio Napolitano

The littoral (nearshore) ecosystem of Lake Tanganyika is the freshwater analog of a coral reef. The clear, shallow waters at the lake’s edge are low in nutrients, but the attached algae (periphyton or microphytobenthos) are highly productive and support the diverse fish community. Dr. Yvonne Vadeboncoeur (Department of Biological Sciences, Wright State University) and Dr. Peter McIntyre (Center for Limnology, University of Wisconsin) are studying the relationships between fish abundance, periphyton productivity and nutrient upwelling in Lake Tanganyika. They are exploring the key roles that grazing and nutrient excretion by fish play in maintaining high ecosystem production.

Lake Tanganyika

Lake Tanganyika

For years, lake ecologists have assumed that phytoplankton, or algae in the water column, are the main source of primary production in lakes. However, evidence is accumulating that, in lakes of all sizes, periphyton forms the energetic base of littoral food webs. Furthermore, the vast majority of fish species in lakes feed in the littoral zone. In doing so, they rely on periphyton for energy, either through direct consumption, or by consuming invertebrates that graze on periphyton. In Lake Tanganyika and other African Rift Valley lakes, the majority of the fish species are grazers and consume periphyton directly. Therefore, periphyton productivity is critical to understanding littoral ecosystem dynamics.

But quantification of the role of periphyton in the lake ecosystem has been hampered by the logistical constraints of measuring periphyton productivity. Chamber methods for measuring phytoplankton productivity are well established and usually include the use of 14C to measure carbon uptake. In contrast, benthic primary productivity is not a routine measurement in lakes. Initial methods also used 14C, but it became increasingly obvious that benthic biofilms have high productivity rates. The high photosynthesis rates made bulk oxygen exchange methods a viable alternative to tracer methods.

The majority of Lake Tanganyika’s fishes, like this Tropheus duboisi, live along the lake’s well-lit shoreline boasting one of the world’s most diverse freshwater communities. 

The majority of Lake Tanganyika’s fishes, like this Tropheus duboisi, live along the lake’s well-lit shoreline boasting one of the world’s most diverse freshwater communities.

Periphyton on rocks or intact sediment cores are easily isolated in chambers. Large changes in oxygen in light and dark chambers can be detected over short incubation periods (15 minutes to 2 hours). The tricky part is getting an accurate oxygen measurement while, at the same time, preventing exchange with the atmosphere. Agitation of the sample introduces error. But stirring is not required to make accurate measurements of oxygen with some DO probes, which has increased both the speed and repeatability of the benthic primary productivity measurements.

Intact periphyton communities are incubated in chambers, either in situ or under lights in the lab. Immediately before withdrawing a water sample, the water is stirred in the chamber with a built-in rotatable paddle or the entire chamber is rotated to mix the water. No air is in the chamber, so this procedure breaks down any oxygen gradients without introducing air that would affect the readings. Water samples are then withdrawn using a disposable, needle-less, 60 cc syringe. The entire syringe is filled with water, and extreme care is used to prevent the introduction of any air into the syringe. After the syringe is filled, it is capped with a small rubber cap.

When the diver returns to the surface, the internal plunger of the syringe is carefully removed, and replaced with the optical-based dissolved oxygen sensor (YSI ProODO). The stainless steel probe guard that protects the optical sensor fits perfectly inside the syringe, and holds the sensor in the center of the water sample. In approximately 40 seconds, researchers have an accurate oxygen measurement. This technique is very fast and allows for many more measurements than previous methods. The ability to make a high number of replicate measurements addresses the common concern of high spatial heterogeneity in periphyton biofilms.

By measuring benthic primary productivity and nutrient uptake in Lake Tanganyika, researchers are beginning to understand how this ecosystem supports so many fish. The fish persistently graze the periphyton, and the water itself is extremely nutrient poor. The biomass of the periphyton is incredibly low, but data from the measurements demonstrates that the algae on the rocks are incredibly productive. How does such high productivity and diversity persist in the face of apparent extreme nutrient scarcity? Perhaps the answer lies in the grazing fish themselves. Most of the nutrients in the system are actually in the bodies of the fish. By staying in the surface waters and feeding on algae, the fish keep the nutrients in the lighted waters where the algae can use them. The fish continually excrete small amounts of nutrients as waste products, and this helps maintain high algal productivity. Ongoing lab and field experiments are helping us understand the mechanisms underlying the high ecosystem production and high fish diversity in Lake Tanganyika.

Waterline thanks YSI for permission to adapt and reprint this article.